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big dye® terminator v1.1 cycle sequencing kit (abi prism)  (Thermo Fisher)


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    Thermo Fisher big dye® terminator v1.1 cycle sequencing kit (abi prism)
    Big Dye® Terminator V1.1 Cycle Sequencing Kit (Abi Prism), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prism+dye+terminator+cycle+sequencing+kit/pm40333311-83-5-12
    Average 90 stars, based on 1 article reviews
    big dye® terminator v1.1 cycle sequencing kit (abi prism) - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: Lipoteichoic acids influence cell shape and bacterial division of Streptococcus suis serotype 2, but play a limited role in the pathogenesis of the infection.
    Article Snippet: Oligonucleotide primers (Table 2) were obtained from Integrated DNA Technologies (Coralville, IA, USA) and PCRs carried out with the iProof proofreading DNA polymerase (BioRad Laboratories, Mississauga, ON, Canada) or Taq DNA polymerase (Qiagen). .. Amplification products were purified using the QIAquick PCR Purification Kit (Qiagen) and sequenced using an ABI 310 Automated DNA Sequencer and ABI PRISM Dye Terminator Cycle Sequencing Kit (Applied Biosystems, Carlsbad, CA, USA). ..

    Article Title: Lipoteichoic acids influence cell shape and bacterial division of Streptococcus suis serotype 2, but play a limited role in the pathogenesis of the infection
    Article Snippet: Oligonucleotide primers (Table ) were obtained from Integrated DNA Technologies (Coralville, IA, USA) and PCRs carried out with the iProof proofreading DNA polymerase (BioRad Laboratories, Mississauga, ON, Canada) or Taq DNA polymerase (Qiagen). .. Amplification products were purified using the QIAquick PCR Purification Kit (Qiagen) and sequenced using an ABI 310 Automated DNA Sequencer and ABI PRISM Dye Terminator Cycle Sequencing Kit (Applied Biosystems, Carlsbad, CA, USA). ..

    Article Title: Study of the Role of Lipoprotein Maturation Enzymes in the Pathogenesis of the Infection Caused by the Streptococcus suis Serotype 2 Sequence Type 25 North American Prototype Strain
    Article Snippet: Oligonucleotide primers ( ) were obtained from Integrated DNA Technologies (Coralville, IA, USA) and PCRs carried out with iProof proofreading DNA polymerase (BioRad Laboratories, Mississauga, ON, Canada) or Taq DNA polymerase (Qiagen). .. Amplification products were purified using the QIAquick PCR Purification Kit (Qiagen) and sequenced using an ABI 310 Automated DNA Sequencer and ABI PRISM Dye Terminator Cycle Sequencing Kit (Applied Biosystems, Carlsbad, CA, USA). ..

    Purification:

    Article Title: Lipoteichoic acids influence cell shape and bacterial division of Streptococcus suis serotype 2, but play a limited role in the pathogenesis of the infection.
    Article Snippet: Oligonucleotide primers (Table 2) were obtained from Integrated DNA Technologies (Coralville, IA, USA) and PCRs carried out with the iProof proofreading DNA polymerase (BioRad Laboratories, Mississauga, ON, Canada) or Taq DNA polymerase (Qiagen). .. Amplification products were purified using the QIAquick PCR Purification Kit (Qiagen) and sequenced using an ABI 310 Automated DNA Sequencer and ABI PRISM Dye Terminator Cycle Sequencing Kit (Applied Biosystems, Carlsbad, CA, USA). ..

    Article Title: Making the most of mortalities: Novel host-parasite records in a sandy inland mouse (Pseudomys hermannsburgensis)
    Article Snippet: Extracted cestode DNA was amplified at two mitochondrial gene regions, the small subunit of ribosomal RNA (rrnS) as per Trachsel et al. (2007), and the cytochrome c oxidase subunit 1 (cox1) as per Bowles et al. (1992) followed by electrophoresis using a 1.5% w/v agarose gel (Table S1). .. Resultant bands of the expected size (~227bp and ~440bp, respectively) were then purified and sequenced in both directions using an ABI Prism Dye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) as per manufacturer instructions. .. 2.4 Sarcoptes scabiei PCR DNA was extracted from the frozen skin sample using a DNeasy blood and tissue kit (QIAGEN) according to manufacturer instructions following a 1-3 hr Proteinase K digestion at 56°C.

    Article Title: Lipoteichoic acids influence cell shape and bacterial division of Streptococcus suis serotype 2, but play a limited role in the pathogenesis of the infection
    Article Snippet: Oligonucleotide primers (Table ) were obtained from Integrated DNA Technologies (Coralville, IA, USA) and PCRs carried out with the iProof proofreading DNA polymerase (BioRad Laboratories, Mississauga, ON, Canada) or Taq DNA polymerase (Qiagen). .. Amplification products were purified using the QIAquick PCR Purification Kit (Qiagen) and sequenced using an ABI 310 Automated DNA Sequencer and ABI PRISM Dye Terminator Cycle Sequencing Kit (Applied Biosystems, Carlsbad, CA, USA). ..

    Article Title: Making the most of mortalities: Novel host-parasite records in a sandy inland mouse (Pseudomys hermannsburgensis)
    Article Snippet: .. A resultant band of the expected size (~400bp) was then purified and sequenced in both directions using an ABI Prism Dye Terminator Cycle Sequencing Kit as per manufacturer instructions. .. 2.5 Sequence analysis Jo ur al Pr e-p roo f All nucleotide sequences were compared with published sequences in GenBank (National Center for Biotechnology Information (NCBI)), using the nucleotide basic alignment search tool (BLASTn), and assessed for similarity with described species.

    Article Title: Making the most of mortalities: Novel host-parasite records in a sandy inland mouse ( Pseudomys hermannsburgensis )
    Article Snippet: .. Resultant bands of the expected size (∼227bp and ∼440bp, respectively) were then purified and sequenced in both directions using an ABI Prism Dye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) as per manufacturer instructions. .. DNA was extracted from the frozen skin sample using a DNeasy blood and tissue kit (QIAGEN) according to manufacturer instructions following a 1–3 h Proteinase K digestion at 56 °C.

    Article Title: Making the most of mortalities: Novel host-parasite records in a sandy inland mouse ( Pseudomys hermannsburgensis )
    Article Snippet: .. A resultant band of the expected size (∼400bp) was then purified and sequenced in both directions using an ABI Prism Dye Terminator Cycle Sequencing Kit as per manufacturer instructions. .. All nucleotide sequences were compared with published sequences in GenBank (National Center for Biotechnology Information (NCBI)), using the nucleotide basic local alignment search tool (BLASTn), and assessed for similarity with described species.

    Article Title: Study of the Role of Lipoprotein Maturation Enzymes in the Pathogenesis of the Infection Caused by the Streptococcus suis Serotype 2 Sequence Type 25 North American Prototype Strain
    Article Snippet: Oligonucleotide primers ( ) were obtained from Integrated DNA Technologies (Coralville, IA, USA) and PCRs carried out with iProof proofreading DNA polymerase (BioRad Laboratories, Mississauga, ON, Canada) or Taq DNA polymerase (Qiagen). .. Amplification products were purified using the QIAquick PCR Purification Kit (Qiagen) and sequenced using an ABI 310 Automated DNA Sequencer and ABI PRISM Dye Terminator Cycle Sequencing Kit (Applied Biosystems, Carlsbad, CA, USA). ..

    Polymerase Chain Reaction:

    Article Title: Lipoteichoic acids influence cell shape and bacterial division of Streptococcus suis serotype 2, but play a limited role in the pathogenesis of the infection.
    Article Snippet: Oligonucleotide primers (Table 2) were obtained from Integrated DNA Technologies (Coralville, IA, USA) and PCRs carried out with the iProof proofreading DNA polymerase (BioRad Laboratories, Mississauga, ON, Canada) or Taq DNA polymerase (Qiagen). .. Amplification products were purified using the QIAquick PCR Purification Kit (Qiagen) and sequenced using an ABI 310 Automated DNA Sequencer and ABI PRISM Dye Terminator Cycle Sequencing Kit (Applied Biosystems, Carlsbad, CA, USA). ..

    Article Title: Lipoteichoic acids influence cell shape and bacterial division of Streptococcus suis serotype 2, but play a limited role in the pathogenesis of the infection
    Article Snippet: Oligonucleotide primers (Table ) were obtained from Integrated DNA Technologies (Coralville, IA, USA) and PCRs carried out with the iProof proofreading DNA polymerase (BioRad Laboratories, Mississauga, ON, Canada) or Taq DNA polymerase (Qiagen). .. Amplification products were purified using the QIAquick PCR Purification Kit (Qiagen) and sequenced using an ABI 310 Automated DNA Sequencer and ABI PRISM Dye Terminator Cycle Sequencing Kit (Applied Biosystems, Carlsbad, CA, USA). ..

    Article Title: Study of the Role of Lipoprotein Maturation Enzymes in the Pathogenesis of the Infection Caused by the Streptococcus suis Serotype 2 Sequence Type 25 North American Prototype Strain
    Article Snippet: Oligonucleotide primers ( ) were obtained from Integrated DNA Technologies (Coralville, IA, USA) and PCRs carried out with iProof proofreading DNA polymerase (BioRad Laboratories, Mississauga, ON, Canada) or Taq DNA polymerase (Qiagen). .. Amplification products were purified using the QIAquick PCR Purification Kit (Qiagen) and sequenced using an ABI 310 Automated DNA Sequencer and ABI PRISM Dye Terminator Cycle Sequencing Kit (Applied Biosystems, Carlsbad, CA, USA). ..

    Sequencing:

    Article Title: Lipoteichoic acids influence cell shape and bacterial division of Streptococcus suis serotype 2, but play a limited role in the pathogenesis of the infection.
    Article Snippet: Oligonucleotide primers (Table 2) were obtained from Integrated DNA Technologies (Coralville, IA, USA) and PCRs carried out with the iProof proofreading DNA polymerase (BioRad Laboratories, Mississauga, ON, Canada) or Taq DNA polymerase (Qiagen). .. Amplification products were purified using the QIAquick PCR Purification Kit (Qiagen) and sequenced using an ABI 310 Automated DNA Sequencer and ABI PRISM Dye Terminator Cycle Sequencing Kit (Applied Biosystems, Carlsbad, CA, USA). ..

    Article Title: Making the most of mortalities: Novel host-parasite records in a sandy inland mouse (Pseudomys hermannsburgensis)
    Article Snippet: Extracted cestode DNA was amplified at two mitochondrial gene regions, the small subunit of ribosomal RNA (rrnS) as per Trachsel et al. (2007), and the cytochrome c oxidase subunit 1 (cox1) as per Bowles et al. (1992) followed by electrophoresis using a 1.5% w/v agarose gel (Table S1). .. Resultant bands of the expected size (~227bp and ~440bp, respectively) were then purified and sequenced in both directions using an ABI Prism Dye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) as per manufacturer instructions. .. 2.4 Sarcoptes scabiei PCR DNA was extracted from the frozen skin sample using a DNeasy blood and tissue kit (QIAGEN) according to manufacturer instructions following a 1-3 hr Proteinase K digestion at 56°C.

    Article Title: Lipoteichoic acids influence cell shape and bacterial division of Streptococcus suis serotype 2, but play a limited role in the pathogenesis of the infection
    Article Snippet: Oligonucleotide primers (Table ) were obtained from Integrated DNA Technologies (Coralville, IA, USA) and PCRs carried out with the iProof proofreading DNA polymerase (BioRad Laboratories, Mississauga, ON, Canada) or Taq DNA polymerase (Qiagen). .. Amplification products were purified using the QIAquick PCR Purification Kit (Qiagen) and sequenced using an ABI 310 Automated DNA Sequencer and ABI PRISM Dye Terminator Cycle Sequencing Kit (Applied Biosystems, Carlsbad, CA, USA). ..

    Article Title: Making the most of mortalities: Novel host-parasite records in a sandy inland mouse (Pseudomys hermannsburgensis)
    Article Snippet: .. A resultant band of the expected size (~400bp) was then purified and sequenced in both directions using an ABI Prism Dye Terminator Cycle Sequencing Kit as per manufacturer instructions. .. 2.5 Sequence analysis Jo ur al Pr e-p roo f All nucleotide sequences were compared with published sequences in GenBank (National Center for Biotechnology Information (NCBI)), using the nucleotide basic alignment search tool (BLASTn), and assessed for similarity with described species.

    Article Title: Making the most of mortalities: Novel host-parasite records in a sandy inland mouse ( Pseudomys hermannsburgensis )
    Article Snippet: .. Resultant bands of the expected size (∼227bp and ∼440bp, respectively) were then purified and sequenced in both directions using an ABI Prism Dye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) as per manufacturer instructions. .. DNA was extracted from the frozen skin sample using a DNeasy blood and tissue kit (QIAGEN) according to manufacturer instructions following a 1–3 h Proteinase K digestion at 56 °C.

    Article Title: Making the most of mortalities: Novel host-parasite records in a sandy inland mouse ( Pseudomys hermannsburgensis )
    Article Snippet: .. A resultant band of the expected size (∼400bp) was then purified and sequenced in both directions using an ABI Prism Dye Terminator Cycle Sequencing Kit as per manufacturer instructions. .. All nucleotide sequences were compared with published sequences in GenBank (National Center for Biotechnology Information (NCBI)), using the nucleotide basic local alignment search tool (BLASTn), and assessed for similarity with described species.

    Article Title: Morphological and Molecular Characterization of a New Isospora (Apicomplexa: Eimeriidae) Species From a Singing Honeyeater ( Gavicalis virescens Vieillot, 1817) (Passeriformes: Meliphagidae) in Western Australia
    Article Snippet: .. This sequencing was conducted using an ABI Prism Dye Terminator Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA), strictly following the manufacturer's protocols as outlined by Yang et al. ( ). ..

    Article Title: Study of the Role of Lipoprotein Maturation Enzymes in the Pathogenesis of the Infection Caused by the Streptococcus suis Serotype 2 Sequence Type 25 North American Prototype Strain
    Article Snippet: Oligonucleotide primers ( ) were obtained from Integrated DNA Technologies (Coralville, IA, USA) and PCRs carried out with iProof proofreading DNA polymerase (BioRad Laboratories, Mississauga, ON, Canada) or Taq DNA polymerase (Qiagen). .. Amplification products were purified using the QIAquick PCR Purification Kit (Qiagen) and sequenced using an ABI 310 Automated DNA Sequencer and ABI PRISM Dye Terminator Cycle Sequencing Kit (Applied Biosystems, Carlsbad, CA, USA). ..



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